DEPARTMENT OF CLINICAL AND EXPERIMENTAL EMBRYOLOGY AND ANDROLOGY,ROYAN INSTITUTE, TEHRAN, IRAN
Abstract
Introduction: The aim of this study was to evaluate the effect of sperm chromatin anomalies on fertilization outcome post ICSI. Material and Methods: Therefore along with semen parameters, CMA3 staining for protamine deficiency, aniline blue staining for excessive histones, SDS for sperm chromatin stability and SDS+EDTA for ability of sperm to undergo decondensation were carried out on 55 semen samples from patients referring for ICSI to Isfahan fertility and infertility cente. Result: The results showed that among the aforementioned tests and semen parameters, only CMA3 showed a significant correlation with fertilization outcome post ICSI. Patients were also grouped according to CMA3 level of <30% or >30 % or fertilization rate of <50%, or >50%. The results showed that the mean percentage fertilization and mean percentage of CMA3 positivity is different inboth groups respectively. The area under ROC curve showed that CMA3 is a highly sensitive and specific test for prediction of fertilization outcome post ICSI. Conclusion: Therefore, it can be concluded that sperm protamine deficiency has profound effect on fertilization failure in ICSI.
Nasr esfahani, M., Razavi, S., Mardani, M., Mafi, A., & Moghadam, A. (2004). Effect Of Human Sperm Chromatin Anomalies On Fertilization Outcome Post ICSI. Cell Journal (Yakhteh), 6(1), 43-49.
MLA
M.H. Nasr esfahani; Sh Razavi; M Mardani; A Mafi; A Moghadam. "Effect Of Human Sperm Chromatin Anomalies On Fertilization Outcome Post ICSI". Cell Journal (Yakhteh), 6, 1, 2004, 43-49.
HARVARD
Nasr esfahani, M., Razavi, S., Mardani, M., Mafi, A., Moghadam, A. (2004). 'Effect Of Human Sperm Chromatin Anomalies On Fertilization Outcome Post ICSI', Cell Journal (Yakhteh), 6(1), pp. 43-49.
VANCOUVER
Nasr esfahani, M., Razavi, S., Mardani, M., Mafi, A., Moghadam, A. Effect Of Human Sperm Chromatin Anomalies On Fertilization Outcome Post ICSI. Cell Journal (Yakhteh), 2004; 6(1): 43-49.
', './data/cellj/coversheet/cover_en.jpg'))