TY - JOUR ID - 248673 TI - Effect Of Human Sperm Chromatin Anomalies On Fertilization Outcome Post ICSI JO - Cell Journal (Yakhteh) JA - CELLJ LA - en SN - 2228-5806 AU - Nasr esfahani, M.H. AU - Razavi, Sh AU - Mardani, M AU - Mafi, A AU - Moghadam, A AD - DEPARTMENT OF CLINICAL AND EXPERIMENTAL EMBRYOLOGY AND ANDROLOGY,ROYAN INSTITUTE, TEHRAN, IRAN AD - Y1 - 2004 PY - 2004 VL - 6 IS - 1 SP - 43 EP - 49 KW - ICSI KW - Sperm Chromatin KW - CMA3 KW - Protamine Deficiency DO - N2 - Introduction: The aim of this study was to evaluate the effect of sperm chromatin anomalies on fertilization outcome post ICSI.Material and Methods: Therefore along with semen parameters, CMA3 staining for protamine deficiency, aniline blue staining for excessive histones, SDS for sperm chromatin stability and SDS+EDTA for ability of sperm to undergo decondensation were carried out on 55 semen samples from patients referring for ICSI to Isfahan fertility and infertility cente.Result: The results showed that among the aforementioned tests and semen parameters, only CMA3 showed a significant correlation with fertilization outcome post ICSI. Patients were also grouped according to CMA3 level of <30% or >30 % or fertilization rate of <50%, or >50%. The results showed that the mean percentage fertilization and mean percentage of CMA3 positivity is different inboth groups respectively. The area under ROC curve showed that CMA3 is a highly sensitive and specific test for prediction of fertilization outcome post ICSI.Conclusion: Therefore, it can be concluded that sperm protamine deficiency has profound effect on fertilization failure in ICSI. UR - https://www.celljournal.org/article_248673.html L1 - https://www.celljournal.org/article_248673_2a801a28138eef65a93cfad4f19a93ad.pdf ER -