In Vitro Expression Of BDNF GDNF NGF NT3 And NT4/5 Genes In Selegiline Induced Bone Marrow Stromal Cells

Document Type : Original Article

Authors

BIOLOGY DEPARTMENT, DAMGHAN UNIVERSITY OF BASIC SCIENCES, DAMGHAN, IRAN

Abstract

Objective: Two types of stem cells can be found in the bone marrow: hematopoietic stem cells and marrow stromal cells (MSCs). If it were possible to induce the differentiation of bone marrow stromal cells into neural cells in vitro and subsequently transplant them into the brain this might help repair lesions observed in some neurodegenerative disorders such as Parkinson’s disease (PD).
Materials and Methods: In this study cultured MSCs were incubated in serum free medium containing 10-8 M selegiline for 24 hours and cells were cultured for another 48 hours in α minimal essential medium (α-MEM) containing 20% fetal bovine serum (FBS). We immunostained selegiline-treated cells for neuronal markers such as NF-200 and TH.
Results: Cell counting results indicated that Selegiline at doses of 10-6 10-7 and 10-8 M in comparison to other doses increased the mean percent of viable cells. The most effective dose of Selegiline for differentiation of bone marrow stromal cells (BMSCs) was 10-8 M. Molecular study indicated that the expression BDNF GDNF NGF NT3 NT4/5 genes was increased in Selegiline-treated cells in comparison to non-treated group.
Conclusion: BMSCs can be directed to a neural fate in vitro and can be considered as a cell source in neurological disorders for autograft therapy.

Keywords

Cell Journal (Yakhteh)
Volume 11, Issue 4
January 2010
Pages 400-407

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