Interference of Bisphenol A on Cumulus Cells Development and Number of Retrieved Mature Oocytes in Unexpected Poor Ovarian Response Women: A Prospective Cohort Study

Document Type : Original Article


1 Department of Biology, Science, and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

3 Breast Disease Research Center (BDRC), Tehran University of Medical Science, Tehran, Iran

4 Department of Obstetrics and Gynecology, Arash Women’s Hospital, Tehran University of Medical Sciences, Tehran, Iran

5 Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


Objective: This study aimed to investigate the relationship between follicular fluid Bisphenol A (BPA) concentrations
with alterations in the expressions of NOTCH1-3, CASPASE 3/7, HLA-G, and ICAM-1 genes and the number of
retrieved mature oocytes (MII oocyte) in the cumulus cells of infertile poor ovarian response stimulates women.
Materials and Methods:In this prospective cohort study, 80 infertile unexpected poor ovarian response (POR) subjects
were selected on the basis of subgroup 1a of the POSEIDON classification. They were divided into two groups: group
1 consisted of 40 women, each with a higher number of metaphase II (MII) oocytes (G1, 3-4 oocytes retrieved), while
group 2 comprised of 40 women, each with a lower number of MII oocytes (G2, ≤2 oocytes retrieved). The expressions
of the studied genes were evaluated by quantitative-real time polymerase chain reaction (PCR). The concentration of
BPA in follicular fluid was measured with HPLC.
Results: The expression levels of NOTCH1-3, HLA-G, and ICAM-1 genes were significantly lower in G2 than G1
(P<0.05). Meanwhile, CASPASE 3/7 expression levels were higher in unexpected POR patients in G2 compared to
G1 (P<0.05). There was a significant direct correlation between the levels of NOTCH1-3, HLA-G and ICAM-1 gene
expressions and there was also a significant inverse correlation (P<0.05) between the levels of CASPASE 3/7, with the
number of MII oocytes and embryo development between the two groups. The concentration of BPA in the follicular
fluids of G2 was higher compared to G1 (P<0.05).
Conclusion: A higher concentration of BPA was associated with a lower number of mature oocytes and oocyte quality
in these patients. Also, alterations of NOTCH1-3, CASPASE 3/7, HLA-G, and ICAM-1 transcript levels in unexpected
POR women were associated with BPA concentration.


Main Subjects

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