Document Type : Original Article
Authors
1
Department of Nephrology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, 046000, China
2
Department of Nephrology Heji Hospital Affiliated to Changzhi Medical College, Changzhi, 046000, China
3
Department of Hematology and Oncology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, 046000, China
4
Department of Endocrinology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, 046000, China
5
Clinical Medicine, Changzhi Medical College, Changzhi, 046000, China
6
Akshita College of Pharmacy
10.22074/cellj.2024.2017978.1468
Abstract
Objective: Chronic inflammation is a common feature in diabetes, especially when blood sugar levels are poorly controlled. This chronic low-grade inflammation can affect various organs, including the kidneys. Podocyte damage play a key role in the development of diabetic nephropathy (DN). The current experimental study was conducted to examine the renal protective effect of Boeravinone B (BB) against streptozotocin (STZ)-induced DN in Sprague-Dawley (SD) rats.
Methods: In order to induce DM in the rats, STZ (60 mg/kg) was administered intraperitoneally, while BB (2.5, 5 and 7.5 mg/kg) was administered orally. The glucose level, body weight and organ weight (hepatic and renal) were also assessed. The renal, histomorphological, antioxidant, hepatic and cytokine levels were estimated. We also estimated the mRNA expression of JAK2 and STAT3. The rats were sacrificed and renal tissues were processed for histopathological analysis.
Results: BB treatment remarkably repressed the glucose level and increased the body weight. BB treatment also suppressed the hepatic weight and augmented the renal tissue weight along with suppressing renal parameters like uric acid, urea, bilirubin, creatinine and albumin. BB treatment significantly (P<0.001) declined the level of histo-morphological parameters such as kidney hypertrophy index (KIH), mean glomerular volume (MGV), foot process fusion ratio (FPFR), and glomerular basement membrane thickness (GBMT). BB treatment remarkably improved the level of renal podocin, renal CD2AP (CD2 associated protein) and suppressed the level of hepatic parameters. BB treatment significantly (P<0.001) altered the level of antioxidant parameters and cytokines. BB treatment also suppressed the mRNA expression of JAK2, STAT3, RAGE, KIM-1, NAGL and S100A8.
Conclusion: BB exhibited the renal protection against STZ induced DN rats via alteration of JAK2/STAT3 signaling pathway.
Keywords
Main Subjects
', './data/cellj/coversheet/cover_en.jpg'))