Document Type : Original Article
Authors
1
Farzanegan Campus, Semnan University, Semnan, Iran.
2
Farzanegan campus, Semnan university, Semnan, Iran.
3
Genetics Departmant tarbiat modares university tehran iran
4
Vali-e-asr reproductive research center, Family research institute, Tehran university of medical science, Tehran, Iran.
10.22074/cellj.2024.2019584.1484
Abstract
Objective: The discovery of novel microRNAs (miRNAs) within essential genes, such as OCT4, signifies a critical step in unraveling gene function. MiRNAs, small non-coding RNA molecules, exert post-transcriptional regulation, impacting cellular processes. Stem cells, crucial for development, depend on precise gene regulation. OCT4, encoding a pivotal transcription factor, is indispensable for stem cell self-renewal, embryonic development, and the maintenance of pluripotency.
Methods and results: In this experimental study, novel microRNA (miRNA) located in OCT4 were bioinformatically predicted and experimentally verified. In order to achieve this, the stem-loop structures inside the OCT4 gene's genomic region were predicted using the SSC profiler web tool. The projected miRNA and its precursor sequence were examined for conservation using the UCSC Genome Browser database. Moreover, the MatureBayes web tool was utilized to predict the mature sequences of the anticipated miRNAs. Additionally, the secondary structure of miRNA was predicted using the RNAfold website. MiRNAseq data analysis confirmed the expression of the predicted miRNA sequence in 64 human tissues. The expression level of predicted miRNA was confirmed in human U87, 1321N1 and A172 cell lines and Glioma, Breast and Lung tissues.
Conclusion: Bioinformatics analysis, microRNA sequencing result and sequencing result of expression study in human cell lines and tissues demonstrate that the OCT4 gene has one miRNA that located in the third exon of the OCT4 gene. The presence of novel miRNA encoded within the OCT4 gene can explain part of the complexities of the OCT4 gene's function.
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