Document Type : Original Article
Authors
1
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
2
Applied Biotechnology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
3
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
10.22074/cellj.2024.2006763.1357
Abstract
Purpose:
The relationship between oxidative stress (OS), insulin resistance (IR), and polycystic ovary syndrome (PCOS) is an important medical issue in human reproduction. Some of the oxidative phosphorylation (OXPHOS) genes have been previously studied in granulosa and muscle cells of PCOS patients. Cumulus cells (CCs) remain close to the oocyte even after ovulation.
This experimental research has been designed to compare the expression of OXPHOS genes in CCs of PCOS, with or without insulin resistance.
Methods:
Patients were included in PCOS insulin-resistant (IR), PCOS insulin-sensitive (IS), and control (fertile women with male infertility history) groups. The expression of NCF2, TXNIP, UCP2, NDUFB6, ATP5H, COX7C, NDUFA3, SDHA, and SDHB was studied by Real-Time PCR, and normalization was performed considering HPRT1 and CYC1 as reference genes. One-way ANOVA and Tukey test were used for data analysis. (P<0.05 was regarded as statistically significant).
Results:
The results showed that the expression of NCF2, TXNIP, UCP2, and ATP5H was significantly higher in the IR group than IS and control groups (P≤0.01). NDUFB6 showed the highest expression in the IS group, which was significantly different from other groups (P≤0.01). The other genes of interest, except COX7C, were observed with the most transcriptional levels in the IS group, although there was no significant difference for those genes.
Conclusions:
Altered expression of genes involved in mitochondrial function compared to the control group in CCs of both IR and IS categories of the PCOS patients suggests that alteration in OXPHOS genes can contribute to the pathophysiology of PCOS.
Keywords
Main Subjects
', './data/cellj/coversheet/cover_en.jpg'))