Upregulation of Oxidative Phosphorylation Genes in Cumulus Cells of The Polycystic Ovary Syndrome Patients with or without Insulin Resistance

Document Type : Original Article


1 Applied Biotechnology Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

2 Department of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

3 International Faculty, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

4 Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

5 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

6 Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran


Objective: The relationship between oxidative stress (OS), insulin resistance (IR), and polycystic ovary syndrome
(PCOS) is an important medical issue in human reproduction. Some of the oxidative phosphorylation (OXPHOS) genes
have been previously studied in granulosa and muscle cells of PCOS patients. Cumulus cells (CCs) remain close to the
oocyte even after ovulation. This research has been designed to compare the expression of OXPHOS genes in CCs of
PCOS, with or without insulin resistance.
Materials and Methods: In this experimental study, patients were included in PCOS insulin-resistant, PCOS insulinsensitive
(IS), and control (fertile women with male infertility history) groups. The expression of NCF2, TXNIP, UCP2,
NDUFB6, ATP5H, COX7C, NDUFA3, SDHA, and SDHB was studied by real-time polymerase chain reaction (PCR),a
and normalization was performed considering HPRT1 and CYC1 as reference genes. One-way ANOVA and Tukey test
were used for data analysis.
Results: The results showed that the expression of NCF2, TXNIP, UCP2, and ATP5H was significantly higher in the
IR group than IS and control groups (P<0.01). NDUFB6 showed the highest expression in the IS group, which was
significantly different from other groups (P<0.01). The other genes of interest, except COX7C, were observed with the
most transcriptional levels in the IS group, although there was no significant difference for those genes.
Conclusion: Altered expression of genes involved in mitochondrial function compared to the control group in CCs
of both IR and IS categories of the PCOS patients suggests that alteration in OXPHOS genes can contribute to the
pathophysiology of PCOS.


Main Subjects

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