Document Type : Original Article
Authors
1
Department of Cell and Molecular Biology Science, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran
2
Department of Cell and Molecular Biology Science, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
3
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
4
Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. 4. Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR,
10.22074/cellj.2024.2022348.1503
Abstract
Background: Endometriosis as a common inflammatory chronic disease is characterized as endometrial tissue growth outside the uterine cavity. It has been reported that lipopolysaccharides (LPS) activate a transcription factor called LPS-induced tumor necrosis factor-alpha (LITAF) in macrophages, which induces transcription of cytokine genes such as tumor necrosis factor alpha (TNF-α). B-cell lymphoma 6 protein (BCL6) is a transcription factor which expression was increased in endometrial tissues of infertile women with endometriosis. In addition, it was shown that mRNA and protein of LITAF and BCL6 were inversely related in mature B lymphocytes and B-Cell lymphomas. Accordingly, we investigated gene expression of LITAF, BCL6 and TNF-α in eutopic and ectopic endometrial tissues of women with endometriosis in compare to controls.
Study Design: In this case control study, ten women with endometriosis (endometriosis group) and ten women without endometriosis (control group) were enrolled after diagnostic laparoscopy. Real-time PCR technique was used for quantitative gene expression. One-Way ANOVA was used for data analysis.
Results: This study showed that LITAF gene expression was significantly higher in ectopic endometrial tissues compared with control samples. The expression level of BCL6 gene was significantly increased in ectopic tissues of women with endometriosis compared with control and eutopic samples. Although TNF-ɑ gene expression was increased in ectopic lesions compared to eutopic and control endometrial samples but these differences were not significant.
Conclusion: The results suggest that over-expression of these inflammatory genes in ectopic endometrial lesions can be considered as a molecular scenario in pathophysiology of endometriosis by induction of inflammatory cascades and cellular proliferation.
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