Resveratrol Nanoformulation Inhibits Invasive Breast Cancer Cell Growth through Autophagy Induction: An In Vitro Study

Document Type : Original Article


1 Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

2 Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran

3 Department of Tissue Engineering, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran


Objective: The aim of this study was to synthesize chitosan nanoparticles (Cs NPs) for resveratrol (RSV) delivery and
assess their effectiveness in inducing autophagy in MDA-MB 231 cells.
Materials and Methods: In this experimental study, Pure and RSV-loaded Cs NPs (RSV. Cs NPs) were prepared via
the ionic gelation method, and their physicochemical properties were characterized using standard techniques, and
RSV release was measured in vitro. MDA-MB 231 cells were incubated with RSV, Cs NPs, and RSV. Cs NPs and
Half-maximal inhibitory concentration (IC50) values were calculated following the MTT test. Cell viability was assessed
by lactate dehydrogenase (LDH) assay, and autophagy was evaluated using the real-time polymerase chain reaction
Results: NP formation was confirmed with the analysis of FTIR spectra. Pure and RSV. Cs NPs had 36.7 and 94.07 nm
sizes with 18.3 and 27 mV zeta potentials, respectively. Above 60% of RSV entrapped within NPs was released in an
initial burst manner followed by a gradual release till 72 hours. Cs and RSV. Cs NPs restrained cell proliferation at lower
concentrations. RSV. Cs NPs showed the highest anticancer effect and stimulated autophagy, indicated by increased
Beclin-1 ATG5, ATG7, LC3A, and P62 expression.
Conclusion: RSV. Cs NPs show promising effects in inhibiting invasive breast cancer (BC) cells in vitro by inducing


Main Subjects


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