Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells on Ovarian Granulosa Cells of Immature NMRI Mice

Document Type : Original Article

Authors

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Biology and Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

Abstract

Objective: In recent years, in vitro maturation (IVM) has become the focus of fertility maintenance, and infertility
treatment. The aim of this study is development of oocytes during folliculogenesis and oogenesis is greatly influenced
by the presence of BMP-7, BMP-15, and GDF-9 genes, which are present in exosomes generated from bone marrow
stem cells.
Materials and Methods: In the experimental study, we investigated how exosomes obtained from bone marrow stem
cells affected development and expansion of ovarian granulosa cells (GCs) in NMRI mice. In this in vitro experiment,
bone marrow stem cells were isolated from mice’s bone marrow, and after identification, exosomes were recovered.
Exosome doses of 100, 50, and 25 μg/ml were applied to GCs before using MTT assay to measure survival rates and
quantitative reverse-transcription polymerase chain reaction (PCR) to measure expression of the BMP-7, BMP-15, and
GDF-9 genes.
Results: The results showed that the GCs treated with exosomes concentrations of 25, 50, and 100 μg/ml significantly
increased bioavailability, growth and proliferation and it also increased expression level of BMP-7, BMP-15 and GDF-9
genes compared to the controls.
Conclusion: Findings of this study indicated that exosomes derived from bone marrow stem cells improved growth
of GCs in NMRI mice and they were a good candidate for further clinical studies to improve quality of the assisted
reproductive techniques.

Keywords

Main Subjects

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