Annexin A7 and Its Related Protein Suppressor of Death Domains Regulates Migration and Proliferation of Hca-P Cells

Document Type : Original Article

Authors

1 School of Pathology, Qiqihar Medical University, Qiqihar, Heilongjiang, China

2 Anesthesia Surgery, The Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang, China

Abstract

Objective: This study was to investigate whether annexin A7 (AnnexinA7, ANXA7) and its co-related protein tumor cell
death domain silencer [suppressor of death domains (SODD)] regulates the migratory phenotype of liver cancer cells.
Materials and Methods: In this experimental study, expression of ANXA7 in Hca-P cells, PANXA7 downregulated
cells and PANXA7 unrelated sequence cells was detected by real-time quantitative polymerase chain
reaction (PCR) at mRNA level and western blotting at protein level. Transwell migration and invasion assays
were performed to determine the migratory phenotype.
Results: After inhibition of ANXA7 expression, expression of SODD protein was also significantly decreased (P<0.05).
Transwell cell transfer experiments showed that number of tumor cells that penetrated into the cell membrane was
significantly reduced after ANXA7 silencing (P<0.05). Transwell cell invasion assay showed that number of tumor
cells penetrating into Matrigel was significantly reduced after ANXA7 down-regulation (P<0.05). The CCK8 assay was
measured at 0, 24 and 48 hours, and proliferation rate of PANXA7 lower weir cells was slower than that of Hca-P cells
and PANXA7 non-related sequence cells (P<0.05).
Conclusion: SODD expression was decreased with the down-regulation of ANXA7. Down-regulating ANXA7 in Hca-P
cells decreased proliferation, migration and invasion of tumor cells.

Keywords

References

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Cell Journal (Yakhteh)
Volume 25, Issue 11 - Serial Number 118
November 2023
Pages 801-808

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