Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis

Document Type : Original Article


1 Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

2 Schools of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran

3 Department of Immunology, Isfahan University of Medical Sciences, Isfahan, Iran

4 Department of Medical Immunology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran


Objective: Rheumatoid arthritis (RA) is a common progressive autoimmune disorder that causes chronic inflammation
of the joints and damage to other organs. Previous studies have reported the important role of miRNA-146a in the
pathogenesis of RA. In addition, the anti-inflammatory and modulatory effects of oleuropein (OLEU) on the expression
pattern of microRNAs (miRNAs) have been shown in different diseases. Therefore, this study aimed to evaluate both
the sensitivity and specificity of miRNA-146a and determine the potential effects of OLEU on the expression levels of
miRNA-146a and tumour necrosis factor-alpha (TNF-α) in RA patients.
Materials and Methods: The participants in this experimental study were divided into 2 groups: RA (n=45) and healthy
controls (n=30). The isolated peripheral blood mononuclear cells (PBMCs) were treated with different concentrations
of OLEU; and the level of TNF-α expression, anti-citrullinated protein, and miRNA-146a were determined using
enzyme-linked immunoassay and real-time polymerase chain reaction, respectively. In addition, the receiver operating
characteristic (ROC) curve analysis evaluated the sensitivity and specificity of miRNA-146a in RA patients.
Results: Results revealed a positive correlation between the levels of miRNA-146a expression with the serum levels of
C-reactive protein (CRP) and rheumatoid factor (RF) in RA patients. In addition, OLEU treatment decreased the levels
of TNF-α and miRNA-146a expression in treated PBMCs samples compared with untreated cells. The ROC curve
analysis showed an 85% sensitivity and 100% specificity of miRNA-146a in RA patients.
Conclusion: Therefore, miRNA-146a can be used as a useful biomarker for RA diagnosis, particularly for early
detection. In addition, OLEU could suppress inflammation in RA patients through the regulation of miRNA-146a.


Main Subjects

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