Document Type : Original Article
Infertility Ward, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Department of Clinical Biochemistry, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
Sleep Disorders Research Centre, Kermanshah University of Medical Sciences, Kermanshah, Iran
Department of Gynaecology and Obstetrics, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Department of Clinical Biochemistry, School of Medicine, Urmia University of Medical Sciences, Urmia Iran
Embryology Laboratory, IVF Department, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
Objective: An association between microRNAs (miRNAs) and adhesion proteins expression with repeated implantation
failure (RIF) has been recently reported; however, these findings are controversial. This study aims to evaluate
the endometrial and circulating expressions of miR-145, miR-155-5p, and miR-224 in addition to the endometrial
expressions of membrane protein palmitoylated-5 (MPP-5) and endothelial cell adhesion molecule-1 (PECAM-1) in
patients with RIF compared to control subjects.
Materials and Methods: This case-control study was carried out between June 2021-July 2022. Subjects included 17
patients with RIF and 17 control subjects, who had previous spontaneous term pregnancy with a live birth, who referred
to the Medical Centre of Arash Hospital, Tehran, Iran. Endometrial tissue samples were obtained via hysteroscopy
and Pipelle catheter in the RIF and control subjects, respectively. Plasma samples were collected after ovulation
in all subjects. The expression levels of MPP5, PECAM-1, miR-224, miR-145, and miR-155-5p were evaluated by
quantitative real-time polymerase chain reaction (qRT-PCR). The student’s t test, chi-square, Mann-Whitney U, and
analysis of covariance (ANCOVA) were used for data analyses.
Results: RIF patients had less endometrial miR-155-5p expression, and higher endometrial and circulating expressions of
miR-145 and miR-224 compared to control subjects. Endometrial PECAM-1 and MPP5 expression significantly decreased in
patients with RIF compared to the control group. There was a positive correlation between circulating miR-224 and endometrial
miR-155-5p, and between circulating miR-155-5p and endometrial PECAM-1 expression levels in patients with RIF.
Conclusion: The present study suggests that circulating miR-224, endometrial miR-145, and PECAM-1 can be
reliable, novel biomarkers for diagnosis of RIF.