Clinical In Vitro Maturationof Human Oocytes



Oocyte maturation is generally defined as the reinitiation of the first meiotic division leading to metaphase II (MII), combined with the appropriate cytoplasmic processes, which are necessary for proper fertilization and early embryo development. Using in vitro maturation (IVM) techniques, oocytes are harvested in the GV stage (germinal vesicle, prophase I), and matured in special maturation media. Especially two major groups of patients may benefit from IVM: One group consists of regularly cycling women with normal ovaries referred for IVF and ICSI due to severe male infertility problems or tubal factor. The other group consists of women suffering from PCO/PCOS. These women are extremely sensitive to stimulation with follicle stimulating hormone (FSH) and have a significant risk of developing ovarian hyperstimulation syndrome (OHSS). An IVM expert meeting in Copenhagen in autumn 2006 came up with recommendations for the treatment of these two groups of Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 29 patients: Inclusion criteria: Age ≤ 35 years BMI 18 – 25 kg/m2 ≤ 3 previously failed IVF cycles ≤ 3 previous IVM attempts No endocrine abnormalities (e.g. hyperprolactinemia, hyperglycaemia, hyperthyroidism) No ovarian cyst>20 mm on cycle day 3 Estradiol level