Document Type : Original Article
.Young Researchers and Elite Club, Najafabad Branch, Islamic Azad University, Najafabad, Iran
.Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran ;.Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
.Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
4.Department of Genetics, Faculty of Modern Medical Science, Islamic Azad University of Medical Sciences of Tehran, Tehran, Iran
5.Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran
6.Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran
7.Department of Biology, Faculty of Natural Sciences and Mathematics, University of Tuzla, Tuzla, Bosnia and Herzegovina
Recent data suggest that increased levels of the HOTAIR long non-coding RNA (lncRNA) are involved in the development of various types of malignancy, including breast cancer. The aim of present study was to investigate HOTAIR lncRNA expression profile in breast cancer (BC) patients and cell lines.
Materials and Methods
In this experimental study, expression level of HOTAIR lncRNA was evaluated in BC and normal tissues of 15 patients as well as MDA-MB-231, MCF-7 and MCF-10A cell lines, using quantitative reverse- transcription polymerase chain reaction (qRT-PCR). HOTAIR lncRNA expression levels were estimated using 2-ΔΔCt method. Further, receiver operating characteristic (ROC) curve analysis was done to evaluate the selected lncRNA diagnostic potential. The Cox’s proportional hazards regression model was performed to evaluate the predictive value of this lncRNA level in BC patients.
The results of present study demonstrated no significant difference in the expression of HOTAIR lncRNA in MCF7 and MDA-MB-231 cancer cell lines compared to MCF-10A as normal cell line (P > 0.05). However, we observed a significantly increase in the expression of HOTAIR in BC patients compared to normal tissues (P < 0.001). Significant associations were found between gene expression and tumour size and margin. We found 91.1% sensitivity and 95.7% specificity of circulating HOTAIR with an area under the ROC curve of 0.969. The Kaplan-Meier analysis indicated significant correlation between HOTAIR expression and overall survival.
This study demonstrated that expression of HOTAIR is increased in BC and might be associated with its progression. According to these findings, HOTAIR expression could be proposed as biomarkers for BC early diagnosis and prognosis.