Preparation and Evaluation of A Novel Liposomal Nano-Formulation in Metastatic Cancer Treatment Studies

Barzegari Firouzabadi, Fatemeh; Oryan, Shahrbanoo; Sheikhha, Mohammad Hasan; Kalantar, Seyed Mehdi; Javed, Ameneh

doi:10.22074/cellj.2019.6008

Preparation and Evaluation of A Novel Liposomal Nano-Formulation in Metastatic Cancer Treatment Studies

Document Type : Original Article

Authors

¹ Department of Animal Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran;Departeman of Biology, College of Science, Payame Noor University, Yazd, Iran

² Department of Animal Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran

³ Reproductive and Genetic Unit, Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

⁴ 4Department of Biology, Faculty of Science, Science and Art University, Yazd, Iran

10.22074/cellj.2019.6008

Abstract

Objective
Today, in clinical trials, we suffer from the lack of effective methods with minimal side effects to deliver medication. Thus, efforts to identify better conditions for delivery of biomedical drugs seem necessary. The purpose of this study was to design a new liposomal formula for transportation of microRNA in osteosarcoma.
Materials and Methods
In this experimental study, several liposomal formulations were synthesized. Physical and chemical parameters, including size, zeta potential, polydispersity index, long-term stability of the liposomal-microRNA complex and the amount of miR-143 loading in liposome based nano-vesicles were optimized using different techniques. Similarly, the effect of free and encapsulated microRNA toxicity were investigated and compared in a human bone osteosarcoma cell line, named SaOs-2.
Results
In this study, we could produce a novel and optimized formulation of cationic PEGylated liposomal microRNA for gene delivery. The present synthesized microRNA lipoplex system was non-agglomerated. The system remained stable after four months and miR-143 leakage was not observed by performing gel electrophoresis. The microRNA lipoplex could enhance conduction of the loaded miR-143, and it also showed good biocompatibility to the healthy cells.
Conclusion
The PEGylated microRNA lipoplex system had a high potential for the systematic migration of miR-143 and it could improve intracellular stability of the released microRNA.

Keywords