The Use Of HepII Cell For Helicobacter Pylori Attachment Assay

Document Type : Original Article

Authors

Department Microbiology , Tehran University of Medical Sciences, Tehran, Iran

Abstract

Introduction: The aim of this study was to determine a proper cell line for adherance of Helicobacter pylori and a suitable method for attachment of this bacteria to cell lines in vitro.
Material and Methods: A total of 22 H.pylori strains,isolated from the antral biopsies of 49 patients with dyspepsia,gastritis,gastric ulcer,duodenal ulcer, etc., were evaluated by ELISA(UPR)to determine the diversity of attachment to 7 mamalian cell lines.
Results: H.pylori can attach to all 7 cell lines,there are no significant differences between 22 H.pylori strains in cell attachment.The attachment pattern of H.pylori to the cells showed a significant reduction respectly from HepII, HeLa, SW742, AGS, HT29/219, HT29 to Caco-2. Maximum attachment was seen to HepII, HeLa and SW742 cells, and among these HepII was the best cell for this purpose. The concentration of H.pylori and cell suspension, condition, and temperature can alter the attachment rate. Best bacterial concentration was equal to 1 Mc farland, and for cell suspension it was 5/Far/*/Lat/104 cells/ml. An incubation period of 90 minutes in 37³C resulted in maximum attachment.
Conclusion: Our study suggests using the introduced method for the study of H.pylori adhesion, attachment, inhibition of attachment and detachment assays and that HepII cell could serve as the best in-vitro model in this regard.

Keywords

Cell Journal (Yakhteh)
Volume 5, Issue 3
September 2003
Pages 177-182

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