Electrospun Poly (Ε-Caprolactone) Nanofiber Mat As Extracellular Matrix

Document Type : Original Article


1 Textile Engineering Department, Isfahan University of Technology, Isfahan, Iran

2 Cell Sciences Research Center, Royan Institute, Stem Cell Department, ACECR, Tehran, Iran

3 Physiology Department, Faculty of Medical Sciences, Isfahan University, Isfahan, Iran


Objective: Investigation of the suitability of electrospun Poly (ε-caprolactone) (PCL) nanofiber scaffold for the Vero cell culture.
Materials and Methods: Electrospinning was used for production of PCL nanofibers 
scaffolds. Scanning electron microscopy (SEM), MTT assay, hematoxylin staining 
and histology analysis were used to investigate the cell morphology, viability, attachment and infilteration of the vero cells on the PCL nanofiber scaffolds.
Results: The results of the MTT assay, SEM images and hematoxylin staining 
showed that Vero cells attach and spread on PCL nanofiber scaffolds. The proliferation of Vero cells is as well as that of control group, but histological analysis showed 
the lack of cell infilteration into the scaffolds, which was found to be due to the small 
diameters of the pores of nanofibrous scaffold.
Conclusion: The result of this study show that PCL nanofiber scaffolds are suitable 
for cell culture, proliferation and attachment and Vero cells attach and proliferate on 
PCL nanofiber scaffolds.