Document Type : Original Article
Department of Biotechnology, Research Institute for Environmental Sciences, International Centre for Science, High Technology and Environmental Sciences, Kerman, Iran
Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
Department of Endodontics, School of Dentistry, Kerman Medical University, Kerman, Iran
Oral and Dental Diseases Research Center, School of Dentistry, Kerman Medical University, Kerman, Iran
Objective: The objective of this study was to isolate and culture human dental pulp stem cells to study important stem cell markers in them.
Materials and Methods: Dental stem cells were isolated from human pulp and cultured in alpha-modified eagle’s medium (α-MEM) supplemented with 20% fetal bovine serum (FBS) in a 37°C incubator with 5% CO2 and photographed under inverted microscope. The expressions of the important stem cell markers were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and agarose gel electrophoresis in the cells at different passages.
Results: Cells isolated from dental pulp showed a high rate of proliferation and were cultured to more than 15 passages in vitro. The study of gene expression by RT-PCR showed that these cells expressed nucleostemin, cyclin D1, Oct-4 and nanog (major components of the PluriNet) in different passages as well as under serum-free conditions.
Conclusion: Cells isolated from dental pulp are genuine pluripotent stem cells with high potential for self-renewal. The expression of the stem cell markers in human dental pulp stem cells indicate that they have a great potential for cell therapy and regenerative medicine, even they were isolated from adult teeth.