A Study Of The Major Components Of The PluriNet Network Expression In Dental Pulp Stem Cells Isolated From Human Third Molar Teeth

Document Type : Original Article


1 Department of Biotechnology, Research Institute for Environmental Sciences, International Centre for Science, High Technology and Environmental Sciences, Kerman, Iran

2 Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

3 Department of Endodontics, School of Dentistry, Kerman Medical University, Kerman, Iran

4 Oral and Dental Diseases Research Center, School of Dentistry, Kerman Medical University, Kerman, Iran


Objective: The objective of this study was to isolate and culture human dental pulp stem cells to study important stem cell markers in them.
Materials and Methods: Dental stem cells were isolated from human pulp and cultured in alpha-modified eagle’s medium (α-MEM) supplemented with 20% fetal bovine serum (FBS) in a 37°C incubator with 5% CO2 and photographed under inverted microscope. The expressions of the important stem cell markers were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and agarose gel electrophoresis in the cells at different passages.
Results: Cells isolated from dental pulp showed a high rate of proliferation and were cultured to more than 15 passages in vitro. The study of gene expression by RT-PCR showed that these cells expressed nucleostemin, cyclin D1, Oct-4 and nanog (major components of the PluriNet) in different passages as well as under serum-free conditions.
Conclusion: Cells isolated from dental pulp are genuine pluripotent stem cells with high potential for self-renewal. The expression of the stem cell markers in human dental pulp stem cells indicate that they have a great potential for cell therapy and regenerative medicine, even they were isolated from adult teeth.