Objective In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo were modeled in vitro. Available healthy sperm obtained in the swim-up procedure using HA were investigated. Materials and Methods This observational cohort study, a routine analysis was conducted on the ejaculation samples obtained from 20 patients. We divided each sample into two groups and the swim-up method was applied. Human serum albumin (HSA, 0.5%) was added to samples from the first group. HA (10%) was added to samples from the second group. We determined the floating linear and non-linear sperm concentrations of both groups annexin V was used to determine the rate of apoptosis of these sperm. Results Following swim-up, linear and non-linear sperm concentrations were higher in the group that contained HA compared to the group with HSA. However, there was a significantly higher apoptosis rate in the HSA group compared to the HA group. Conclusion The addition of HA to the medium in the swim-up procedure positively affected sperm parameters. Thus, healthier sperm cells were obtained without DNA damage and with high motility.
Saylan, A., & Duman, S. (2016). Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method. Cell Journal (Yakhteh), 18(1), 83-88. doi: 10.22074/cellj.2016.3990
MLA
Aslihan Saylan; Selcuk Duman. "Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method". Cell Journal (Yakhteh), 18, 1, 2016, 83-88. doi: 10.22074/cellj.2016.3990
HARVARD
Saylan, A., Duman, S. (2016). 'Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method', Cell Journal (Yakhteh), 18(1), pp. 83-88. doi: 10.22074/cellj.2016.3990
VANCOUVER
Saylan, A., Duman, S. Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method. Cell Journal (Yakhteh), 2016; 18(1): 83-88. doi: 10.22074/cellj.2016.3990