Simultaneous Coating of Electrospun Nanofibers with Bioactive Molecules for Stem Cell Osteogenesis In Vitro

Document Type : Original Article


1 Department of Cell and Molecular Biology, International Campus-Kish, University of Tehran, Kish Island, Iran

2 Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran

3 School of Biotechnology, College of Science, University of Tehran, Tehran, Iran

4 Department of Medical Laboratory Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran


Objective: Mesenchymal stem cells (MSCs) are widely recognized as a promising cell type for therapeutic applications
due to their ability to secrete and regenerate bioactive molecules. For effective bone healing, it is crucial to select a
scaffold that can support, induce, and restore biological function. Evaluating the scaffold should involve assessing MSC
survival, proliferation, and differentiation. The principal aim of this investigation was to formulate composite nanofibrous
scaffolds apt for applications in bone tissue engineering.
Materials and Methods: In this experimental study, nanofibrous scaffolds were fabricated using Poly-L-lactic acid
(PLLA) polymer. The PLLA fibers’ surface was modified by integrating collagen and hydroxyapatite (HA) nanoparticles.
Results: The findings demonstrated that the collagen- and nanohydroxyapatite-modified electrospun PLLA scaffold
positively influenced the attachment, growth, and osteogenic differentiation of MSCs.
Conclusion: Coating the nanofiber scaffold with collagen and nanoparticle HA significantly enhanced the osteogenic
differentiation of MSCs on electrospun PLLA scaffolds.


Main Subjects

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