Document Type : Original Article
Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran
Dental School, Semnan University of Medical Science, Semnan, Iran
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
Objective: Parkinson’s disease (PD) is a neurodegenerative disorder described by the dynamic decline of dopaminergic
neurons in the substantia nigra pars compacta (SNpc). Stem cell transplantation is a new therapeutic strategy in the
treatment of PD. The objective of the study was to assess the impact of intravenous infusion of adipose-derived
mesenchymal stem cells (AD-MSCs) on memory disorder in Parkinsonian rats.
Materials and Methods: In this experimental study, male Wistar rats were randomly divided to four groups containing
sham, cell treatment, control, and lesion. The cell treatment group received intravenous injection of AD-MSCs 12 days
after PD induction by bilateral injection of 6-hydroxydopamine. Four weeks after lesion formation, spatial memory
was examined using the Morris water maze (MWM) assessment. The rats’ brains were removed and assessed by
bromodeoxyuridine (BrdU), tyrosine hydroxylase (TH), and glial fibrillary acidic protein (Gfap) immunostaining.
Results: Statistical analyses revealed a significant addition and reduction in time spent and escape latency in the target
quadrant, respectively, in the cell group as compared to the lesion group. Also, BrdU-labeled cells were present in the
substantia nigra (SN). The density of TH-positive cells was significantly increased in the AD-MSCs transplantation group
as compared to the lesion group, and the density of astrocytes significantly diminished in the AD-MSCs transplantation
group as compared to the lesion group.
Conclusion: It appears that AD-MSCs treatment for Parkinson’s could decrease the density of astrocytes and promote
the density of TH-positive neurons. It appears that AD-MSCs could improve spatial memory impairment in PD.