A Haspin promoter element induces tissue-specific methylation of a transcription region and the regulation of gene expression in mouse ova

Document Type : Short Communication


1 Labaratory of Molecular Biology, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, Japan

2 Department of Genome Editing, Institute of Biomedical Science, Kansai Medical University, Shin-machi, Hirakata City, Osaka, Japan


HASPIN is a nuclear serine-threonine kinase originally identified in the mouse testis. Its 193 bp DNA promoter element
(hereafter, 193PE) regulates bidirectional, synchronous gene expression in the germ cells of male mice. Recent studies
have shown that Haspin is also expressed in trace amounts in somatic cells; HASPIN also functions in oocytes.
Haspin expression is regulated by the tissue-specific methylation of Haspin genomic DNA regions, including somatic
cells. This study investigated relationship between 193PE and DNA methylation by examining methylation status of
transgenic mice carrying 193PE and a reporter gene. In somatic (liver) cells carrying the reporter gene, 193PE induced
methylation as well as trace expression of the reporter gene. In the testis, 193PE induced hypomethylation and intense
reporter gene expression. Expression of HASPIN in an egg was assessed using human chorionic gonadotrophin to
induce ovulation in female transgenic mice. The results showed that 193PE induced tissue-specific methylation, which
resulted in reporter gene expression in a mouse egg.


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