Document Type : Original Article
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Department of Pharmacology and Toxicology, School of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
Experimental and Applied Pharmaceutical Sciences Research Center, Urmia University of Medical Sciences, Urmia, Iran
Objective: Colonic anastomosis is associated with serious complications leading to significant morbidity and mortality.
Fibroblasts have recently been introduced as a practical alternative to stem cells because of their differentiation
capacity, anti-inflammatory, and regenerative properties. The aim of this study was to evaluate the effects of intramural
injection of fibroblasts on the healing of colonic anastomosis in rats.
Materials and Methods: Inbred mature male Wistar rats were used in this experimental study (n=36). Fibroblasts were
isolated from the axillary skin of a donor rat. In the sham group, manipulation on descending colon was done during
laparotomy. A 5 mm segment of the colon was resected, and end-to-end anastomosis was performed. In the control
group, 0.5 ml of phosphate buffer saline (PBS) was injected into the colonic wall and in the treatment group, 1×106
fibroblasts were transplanted. Following euthanasia on day 7, intra-abdominal adhesion, leakage and peritonitis were
evaluated by necropsy. Mechanical properties were assessed using bursting pressure and tensile tests. Inflammation,
angiogenesis, and collagen deposition were examined histopathologically.
Results: The mean scores for adhesion and leakage were decreased in the treatment group versus control samples.
Lower infiltration of inflammatory cells was observed in the treatment group (P=0.03). Angiogenesis and collagen
deposition scores were significantly increased in the fibroblast transplanted group (P=0.03). Tensile mechanical
properties of the colon were significantly increased in the treatment group compared to the control sample (P=0.01).
There was no significant difference between the control and treatment groups in terms of bursting pressure (P=0.10).
Positive weight changes were found in sham and treatment groups, but the control rats lost weight after 7 days.
Conclusion: The results suggested that allotransplantation of dermal fibroblasts could improve the necroscopic, histopathological, and biomechanical indices of colonic anastomosis repair in rats.