The Impact Of Different Cell Culture Mediums On CD8+T Cells Expansion: A Bioinformatics Study

Document Type : Original Article


1 Department of Applied Cell Sciences, Faculty of Basic Sciences and Advanced Medical Technologies, Royan Institute, ACECR, Tehran, Iran

2 Hematopoeitic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

4 Department of Regenerative Biomedicine and Cell Terapy, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran


Objective: Different Cell Culture medias can affect the expansion of T cells. The aim of this study is to assess signaling
pathways, protein interactions and genes in T cells cultured in different common T cell expansion medias to select the
best candidate.
Materials and Methods: In this in silico observational study, with the use of bioinformatics analysis and the use of enrichment databases, gene expression profiles were investigated using microarray analysis.
Results: The results of this study were the joint selection of 26 upregulated genes and 59 downregulated genes that were involved in SREBP control of lipid synthesis, co-stimulatory signal during T-cell activation mitosis and chromosome
dynamics, telomeres, telomerase, and cellular aging signal pathways.
Conclusion: Using bioinformatics analyzes, integrated and regular genes were selected as common genes CD80, LST1, ATM and ITM2B 4-1BBL, Akt inhibitor, interleukin 7 and 15 expansion media. 


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