Upregulation Of Hsa-MiR-625-5p Inhibits Invasion Of Acute Myeloid Leukemia Cancer Cells Through ILK/AKT Pathway

Samieyan Dehkordi, Sahar; Mousavi, Seyed Hadi; Ebrahimi, Marzieh; Alizadeh, Shaban; Hedayati, Amir Abbas; Mohammad, Monireh; Aliabedi, Bahareh

doi:10.22074/cellj.2022.7658

Upregulation Of Hsa-MiR-625-5p Inhibits Invasion Of Acute Myeloid Leukemia Cancer Cells Through ILK/AKT Pathway

Document Type : Original Article

Authors

¹ Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran

² Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

10.22074/cellj.2022.7658

Abstract

Objective: Acute myeloid leukemia (AML) is characterized by abnormalities of differentiation and growth of primary hematopoietic stem cells (HSCs) in the blood and bone marrow. In many studies, miR-625-5p has been shown to inhibit
downstream pathways from affecting the metastasis and invasion of the integrin-linked kinase (ILK) signaling pathway. It has been proved that the expression of miR-625-5p decreases in AML cell lines. This study aimed to investigate the effect of miR-625-5p upregulation on the invasion of KG1 ell line in vitro.
Materials and Methods: In this experimental study, we investigated the impact of upregulation of miR-625-5p on invasion via the ILK/AKT pathway in the KG1 cell line. After transfection using the viral method, the cellular invasion was assessed by invasion assay and the levels of miR-625-5p genes and protein were evaluated by quantitative polymerase chain reaction (qPCR) and western blotting. Moreover, CXCR4 level was assessed by flow cytometry.
Results: The invasion significantly reduced in MiR-625-5p-transfected KG1 cells (P<0.01) that was concomitant with remarkably decreasing in the expression levels of ILK, NF-κB, and COX2 genes compare with the control group (P<0.01). Incontrast, MMP9, AP1, and AKT significantly increased (P<0.01, P<0.001 and P<0.01, respectively) and GSK3β did not change significantly in MiR-625-5p-transfected KG1 cells. The protein level of NF-κB decreased (P<0.01) and MMP9 increased, however it was not significant. Moreoever, the expression of CXCR4 was significantly lower (P<0.01) in comparison with the control group.
Conclusion: miR-625-5p leads to a reduction in cell invasion in the AML cell line through ILK pathway. Therefore, it could be a breakthrough in future AML-related research. However, further studies are needed to support this argument.

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