Document Type : Original Article
Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Bone Marrow Transplantation Center, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Clinical Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Iran
Objective: Multiple Myeloma (MM) is a heterogeneous cytogenetic disorder in which clonal plasma cells proliferate in the bone marrow (BM) and cause bone destruction. The BM microenvironment plays a crucial role in pathogenesis of this disease, and mesenchymal stem cells (MSCs) are one of the key players. Herein, we propose to investigate the expressions of hsa-MIR-204, runt-related transcription factor 2 (RUNX2), peroxisome proliferator-activated receptor gamma (PPARγ), and B-cell lymphoma 2 (BCL2) as factors involved in osteogenesis, adipogenesis, and MSC survival in BM-MSCs from MM patients and normal individuals.
Materials and Methods: In this experimental study, we isolated MSCs from BM aspirates of MM patients and healthy donors. Total RNA were extracted before and after co-culture with L363 myeloma cells. Gene expressions of RUNX2, PPARγ, BCL2, and hsa-MIR-204 were assessed by quantitive real time polymerase chain reaction (qRT-PCR).
Results: Higher levels of RUNX2, PPARγ, and hsa-MIR-204 expressions existed in MMMSCs compared to normally derived (ND)-MSCs. BCL2 expression decreased in MMMSCs. We observed different results in the co-culture model.
Conclusion: In general, the MM-MSCs gene expression profile differed compared to NDMSCs. Upregulation of RUNX2, PPARγ, and hsa-MIR-204 in MM-MSCs compared to NDMSCs would result in formation of bone defects. Downregulation of BCL2 would lead to MM-MSC cell death.