Letrozole Effect On In VitroCulture Of Human EndometrialExplants In Fibrin Matrix



Introduction: In vitro culture of human endometrial tissue introduced as a model for endometriosis. Drug treatment options in endometriosis are unsatisfactory and more researches on this disease are needed. Aromatase inhibitors are candidate drug in the treatment of endometriosis. The aim of the present study is to investigate an aromatase inhibitor (letrozole) effects on human endometrium in three-dimensional (3D) fibrin matrix.
Materials and Methods: Normal human endometrial biopsies (N=8) from reproductive age women (mean age 28.3 year) were cultured in fibrin matrix for 21 days as a control and different doses of letrozole (0.1, 1 and 10 μM). Tissue changes were evaluated by two systems: an invert microscopic grading system (Marking criteria) and computerized program analysis. Routine histochemical and immunohistochemichal staining were done to document stromal, epithelial and endothelial cells. Data were analyzed by one way ANOVA and post hoc Tukey test.
Results: Letrozole (0.1, 1 and 10 μM) exert a significant (p