Mouse Back Muscle As APromising Site For Human OvarianTissue Xenotransplantation



Introduction: Loss of follicles during initial ischemia constitutes the main limitation of ovarian tissue transplantation (OTT). We investigate the efficiency of the back muscle (B) site for human OTT using a SCID mouse xenografting model.
Materials and Methods: 1) Study of vascularization and integration of OT in the grafting site during the first eight days after transplantation and Immunohistochemical staining (Anti-human and anti-mouse CD31 and CD34) to evaluate neovascularization. 2) Follicular development in B and K xenografts, three, five and seven months after grafting.
Results: 1) Anti-mouse CD34 and CD31 positive endothelial cells were first noticed in B and K grafts on days 3 and 4 respectively. On day 5 all B and 70% of K grafts and on day 8, all B and K grafts were positive for mouse markers, but a higher number of murine blood vessels were counted in the B versus K grafts (14.0±1.3 versus 6.60±0.68, p