Objective: To determine whether varied human spermatozoa detected also with monoclonal antibodies against acrosomal proteins, have an influence on fertilization, transfer, pregnancy and implantation rates using ICSI. Design: A retrospective study. Setting: A private IVF center and academic research laboratory. Patients: 1240 men participating in the ICSI program. Interventions: Sperm were divided into seven groups: oligozoospermia, oligoasthenozoospermia, ligoasthenoteratozoospermia, fresh and frozen/thawed epididymal and fresh and frozen/thawed testicular sperm. Fertilization, transfer, pregnancy and implantation rates were recorded in each category. Sperm were tested with antibodies for detection of the of the sperm acrosome. Main outcome measure: Fertilization, transfer, pregnancy and implantation rates, percentage of acrosome-reacted cells. Results: The fertilization rate and statistical evaluation showed differences between morphologically normal and pathological sperm and other groups. The freezing-thawing procedure had no influence on the fertilization of testicular sperm but epididymal frozen/thawed sperm had a higher fertilization rate. Immunofluorescence proved decreasing sperm quality in all groups compared to the control group. This difference is not manifested in other parameters (transfer, pregnancy, implantation rates). Conclusion(s): The spermatozoa with varied semen characteristics and good quality, also detected with specific antibodies, gave the best fertilization rates. The paternal effect is not proved in other parameters.
O, T., L, P., K, K., J, M., M, M., & F, E. (2007). Evaluation Of ReproductivePotential After IntracytoplasmicSperm Injection Of Varied HumanSemen Tested By AntiacrosomalAntibodies. Cell Journal (Yakhteh), 9(supplement1), -.
MLA
Tepla O; Peknicova L; Koci K; Mika J; Mrazek M; Elzeinova F. "Evaluation Of ReproductivePotential After IntracytoplasmicSperm Injection Of Varied HumanSemen Tested By AntiacrosomalAntibodies". Cell Journal (Yakhteh), 9, supplement1, 2007, -.
HARVARD
O, T., L, P., K, K., J, M., M, M., F, E. (2007). 'Evaluation Of ReproductivePotential After IntracytoplasmicSperm Injection Of Varied HumanSemen Tested By AntiacrosomalAntibodies', Cell Journal (Yakhteh), 9(supplement1), pp. -.
VANCOUVER
O, T., L, P., K, K., J, M., M, M., F, E. Evaluation Of ReproductivePotential After IntracytoplasmicSperm Injection Of Varied HumanSemen Tested By AntiacrosomalAntibodies. Cell Journal (Yakhteh), 2007; 9(supplement1): -.
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