Association between rs11614913 Polymorphism of The MiR-196-a2 Gene and Colorectal Cancer in The Presence of Departure from Hardy-Weinberg Equilibrium

Document Type : Original Article

Authors

1 .Department of Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran;.Modeling of Noncommunicable Diseases Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

2 .Kermanshah Province Electricity Distribution Company, Kermanshah, Iran

3 .Department of Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran;4.Research Center for Health Center, Hamadan University of Medical Sciences, Hamadan, Iran

4 5.Department of Molecular Medicine and Genetics, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

5 6.Gastrointestinal (GI) Cancer Department, Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6 .Department of Biostatistics, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran

Abstract

Objective
Colorectal cancer (CRC) is the fourth most common and the second most lethal cancer worldwide. CRC mortality is increasing in Iran. In the current study, we aimed to investigate association between rs11614913 polymorphism of the miR-196-a2 gene and CRC.
Materials and Methods
In this case-control study, we assessed association of the rs11614913 polymorphism in 194 patients with CRC (case) and 286 healthy individuals (control). The expectation-maximization (EM) algorithm method was used to adjust deviation from Hardy-Weinberg equilibrium (HWE).
Results
There was no significant difference between genotypic frequencies of rs11614913 polymorphism in the control and case groups. Genotypic frequencies differed in the adjusted and unadjusted deviations from the HWE. Analysis of unadjusted and adjusted independent variables showed that age, sex, alcohol consumption, and drug use were statistically significant.
Conclusion
Our findings showed that rs11614913 polymorphism was not associated with CRC risk. Deviation from HWE affected the results. It is recommended to perform further studies to establish HWE. Ignoring the equilibrium can cause in consistencies in the results of studies.

Keywords