Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract
Angiogenesis, the formation of new blood vessels, is a key process in cancer development and metastasis. In this study, the anti-angiogenic and anti-proliferative potentials of Ficus carica latex extract have been investigated using human umbilical vein endothelial cells (HUVECs). Different doses of latex extract were prepared and added to a three-dimensional culture of HUVEC in a collagen matrix. After 3-5 days of treatment, the anti-angiogenic effects of the extracts were monitored microscopically. For the anti-proliferation assay, different doses of the extracts were examined on HUVECs. The results clearly indicated that latex extract could inhibit proliferation and capillary tube formation of HUVECs in a dose-dependent manner at the range of 100-400 μg/ml. In addition, the extract was not cytotoxic up to 450 μg/ml as assessed by trypan blue and lactate dehydrogenase (LDH) cytotoxicity assays. It is concluded that latex extracts of F. carica contain strong anti-angiogenic and anti-proliferative activities. Our data indicates that latex extract could be a candidate as a potential agent for the prevention of angiogenesis in cancer and other chronic disorders.
Mostafaie, A., Mansouri, K., Norooznezhad, A., & Mohammadi-Motlagh, H. (2011). Anti-Angiogenic Activity of Ficus carica Latex Extract on Human Umbilical Vein Endothelial Cells. Cell Journal (Yakhteh), 12(4), 525-528.
MLA
Ali Mostafaie; Kamran Mansouri; Amir-Hossein Norooznezhad; Hamid-Reza Mohammadi-Motlagh. "Anti-Angiogenic Activity of Ficus carica Latex Extract on Human Umbilical Vein Endothelial Cells". Cell Journal (Yakhteh), 12, 4, 2011, 525-528.
HARVARD
Mostafaie, A., Mansouri, K., Norooznezhad, A., Mohammadi-Motlagh, H. (2011). 'Anti-Angiogenic Activity of Ficus carica Latex Extract on Human Umbilical Vein Endothelial Cells', Cell Journal (Yakhteh), 12(4), pp. 525-528.
VANCOUVER
Mostafaie, A., Mansouri, K., Norooznezhad, A., Mohammadi-Motlagh, H. Anti-Angiogenic Activity of Ficus carica Latex Extract on Human Umbilical Vein Endothelial Cells. Cell Journal (Yakhteh), 2011; 12(4): 525-528.
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