Document Type : Original Article
Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium
Laboratory for Protein Biochemistry and Protein Engineering, Ghent, Belgium
Reproductive Biotechnology Research Center, Avicenna Research Institute, Shahid Beheshti University, Tehran, Iran
Objective: Chronic hypoxia exists in many diseases, including cancer. The subject of our study is analysis of molecular pathways affected in the chronically hypoxic mouse brain.
Materials and Methods: Using the emPAI protocol, we performed a quantitative proteomic approach to characterize the global proteome in the mouse brain exposed to 7% O2 for 48 hours.
Results: Utilizing the emPAI protocol to estimate protein abundance and assign molar concentrations to all proteins, we were able to identify 33 proteins with significant changes in their expression.
Conclusion: Deregulated proteins were mainly involved in cell metabolism, apoptosis, Ca2+ signaling, pentose phosphate pathway, 14-3-3 protein mediated signaling cascades and protein degradation. The obtained data will provide some clues for understanding mechanisms with which cells respond and adapt to chronic hypoxia.