The Effect Of SemenParameters And Sperm ChromatinStatus Before And PostVaricoclelctomy On Fertility



Introduction: Different methods have been used to evaluate the beneficial effect of varicocelectomy; these include semen parameters and pregnancy rate. Due to high biological variability of semen parameters, sperm functional tests have been considered as an efficient end point in assessment of fertility. Therefore, the aim of this study was to evaluate the effect of varicocelectomy on semen parameters, sperm chromatin and membrane integrity.
Materials and Methods: In 35 patients over a 2 year period protamine deficiency, presence of excessive histone, chromatin stability, Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 13 ability of sperm to undergo decondensation and membrane integrity were assessed by chromomycin A3, aniline blue staining , SDS, SDS+EDTA and HOST, respectively.
Results: The results of this study showed that among semen parameters only sperm motility, sperm membrane integrity and sperm chromatin integrity showed significant improvement post surgery. The cumulative pregnancy rate in this was 25.7%. Comparing the results of the aforementioned parameters between patients whom became pregnant compared to those who did hot benefit from varicocelectomy show that patient may benefit from varicocelectomy that have improved sperm morphology, decreased sperm protamine deficiency and decreased presence of excessive histone at 3 month post surgery (p