Comparative Effect OfAminoglycosides (Gentamycin,Neomycin, Streptomycin) AndFluoroquinolones (Ofloxacin)Antibiotics On Sperm Parametersand Testis Apoptosis In Rats



Introduction: Aminoglycosides (Gentamycin, Neomycin, Streptomycin) and fluoroquinolones (ofloxacin) are synthetic antibacterial agent antibiotics with a very broad spectrum against microbial pathogens, especially the Gram-negative. The aim of this study was to investigate the comparative effects of these drugs on testis apoptosis and sperm parameters in rats.
Materials and Methods: The fifty male Wistar rats were selected and randomly divided into control (n=10) and experimental (n=40) groups. The experimental groups split into four groups. First, second, third and fourth experimental groups received 5 mg/kg (IP) Gentamycin, 50mg/kg (IP) Neomycin, 40mg/kg (IP) Streptomycin and 72mg/kg (PO) ofloxacin daily for fourteenth day, respectively; however, the control group just received vehicle (IP). In the fourteenth day, rats were killed and sperm removed from cauda epididymis and analyzed for sperm motility, morphology, and viability. Testis tissues were also removed and prepared for TUNNEL assay for detection of apoptosis.
Results: There was a significant decrease in sperm count, viability and motility in all of experimental groups when compared with control group (p