The Effect Of LIF On LowMotility Sperms In Men WithAsthenospermi



Introduction: From the past until now extensive study has been taken to deal with the infertility problems in men. Some of the causes are related to men infertility can be defined as reduced number of sperms abnormal morphology and weakness in motility of sperm. Recently improvement in quality of sperm such as number and morphology by in vitro experiments is not possible at the present time. Improvement in quality of sperm motility by means of increasing motility with drugs is possible. Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 21 Investigations have shown that closed relation exist between sperm motility and fertility rate by adding progesterone, platelet activating factor, follicular fluid, cytokines & pentoxyphilline by in vitro experimentation which increasing the ability of the sperm motility and survival time. On the other hand, some studies has been done on the effect of LIF on normal sperms motility and shown that this factor could improve normal sperm motility. In this project by knowing the idea that there is no report about the effect of LIF on low motility sperms in men with asthenospermi, we decided to determine effect of this factor on low motility sperms.
Materials and Methods: In this investigation the semen sample of 15 infertile men who have already referred to IVF department of Imam Khomeini Hospital were collected and then preparing them by successive stages for culturing in Ham's F-10 with different concentrations 3, 5, 10, 50ng/ml in incubator on the condition 37 , %5 co2 incubated and samples evaluate for motility and survival time at 6, 24, 48 hours, after culturing, collected data with ANOVA & LSD method by means of SPSS software were analyzed.
Results: The results showed that this factor doesn't effect own motility and survival time of sperm with the duration of 6 hours but after 24 hours of culturing in 10ng/ml and 48 hours of culturing in 50ng/ml concentration of LIF respectively the results show improvement in both motility and vitality of sperm.
Conclusion: Our data showed that LIF has positive effects on motility and vitalaity of human sperm in dose dependant.