Ovarian TissueTransplantation: A New Method AndSite For Induction OfFolliculogenesis In Mice As A Modelfor Human Female



Introduction: Ovarian tissue transplantation is a new method of restoring fertility to women whose ovaries are not functioning normally. Young women who undergo chemotherapy or radiotherapy for cancer face serious consequences to their reproductive health and severely affect the ovarian follicular store, especially. Therefore, the aim of this study was to demonstrate induction of the folliculogenesis from ovarian tissue (OT) transplanted under kidney capsule in the presence or absence of gonadotropins support.
Materials and Methods: Forty eight healthy female mice were anesthetized and abdominal cavity is open. From one side of the body, small piece (~1 X 1 X 1 mm) of OT was transplanted to the subcapsular membrane of kidney at another side, and surgical operation is closed. Then, female mice were classified into three groups according to the time of gonadotropins injection. Group-1: mice injected with sterile normal saline (control group). Group-2: mice injected with gonadotropins directly for four days. Group-3: mice injected with gonadotropins for four days after eight days of surgical operation. Follicular growth, quality of retrieved ova and histological changes for transplanted OT were assessed.
Results: In general, no deletion for transplanted OT pieces and no side effects post-operation on mice of all groups were recorded. Best follicular growth of transplanted OT was achieved for groups 1 and 2. Graafian follicles were obtained from transplanted OT of group-2, and less degree for group-1. However, least degree for follicular growth of transplanted OT was reported for group-3 as compared to other groups. Immature and mature oocytes with corona and cumulus cells collected by squashing of transplanted OT.
Conclusion: The present data demonstrate that the ovarian tissue transplantation is possible to undergo follicular growth subcapsular of the kidney. Also, physiology of the body supports the ovarian follicular growth in another site other than normal position. Further studies are recommended on in vitro Abstract of the 8th Royan International Twin Congress, Tehran, Iran, 5-7 September 2007 26 Yakhteh Medical Journal, Vol 9, Sup 1, Summer 2007 maturation and fertilization of retrieved ova and embryo transfer.