The Co-Culture With Vero Cells And The Development Of Two Cell Mouse Embryos After Vitrification



Introduction: The Mouse embryos can successfully be vitrified using ethylene glycol as cryoprotectant, however their development differs significantly from the non-vitrified embryos. The ability of their development can be improved when they co-culture with somatic cells. In the present study, the effects of co-culturing with Vero cells on the development of vitrified two cell mouse embryos were studied.
Materials and Methods: Two cell embryos were flushed from the excised uteri of superovulated mice. Morphologically normal embryos were vitrified using 10% ethylene glycol solution (EFS10), and thawed rapidly using 0.5 M sucrose solution. The survived embryos were divided to two grops: one group cultured on RPMI and the another one co-cultured with Vero cells. Control embryos was considered for each experimental groups.
Results: The survival rate was 83%. The developmental rate of embryos which could reach to morula stage for the exp. group 1 and 2 were 52% and 69% respectively which the difference between the exp. groups was significant (P<0.001). Mean while 48% of the exp. group 1 could form blastocysts, accordingly the second group showed 60% and the difference was significant (P<0.001). The difference between the two exp. groups which could reach to hatching stage was not significant.
Conclusion: The results suggest that the co-culture with Vero cells can improve the development of vitrified two cell mouse embryos