The Effect Of Co-Culture Of Epithelial Cells, Isolated From Ampullary And Isthmic Regions Of Human Oviduct, On 2-Cell Mouse Embryos



Introduction: This study was initiated to examine the effect of the ampullary and the isthmic primary cell cultures of human oviduct during long-term co-culture on two-cell mouse embryos.
Materials and Methods: Using a mechanical procedure, epithelial cells from ampullary (A) and isthmic (I) regions of the human oviduct were isolated and cultured in Ham's F-10 with 10% fetal calf serum (FSC). The 2-cell embryos were prepared from Albino Swiss mice, which were co-cultured in Ham\rquote s F-10 with 10% FSC with either primary A-cell culture or primary I-cell for 5 days. The control group was Albino Swiss mouse embryos which were maintained in the same culture medium without co-culturing.
Results: After 120\super h \nosupersub , the co-cultured embryos groups (A and I) had more blastocyts (4% an 41% respectively) and hatched embryos (22% both) as they were compared with the control which showed 16 and 8 percentages for blastocyts and hatched embryos respectively. The levels of significance were high (P<0.001 both). Moreover, in first 48h, the development rete was more in the embryos which were co-cultured with A-cell than that of I-cell (P<0.001 for 24h and p<0.01).
Conclusion: The results suggest that an unknown factor which may be released the epithelial cell in the co-culture stimulating the development of the mouse embryos. Moreover, the in virto development of embryos may be more supported by human A-cell than human I-cell.