Document Type : Original Article
RADIOLOGY DEPARTMENT, MEDICAL OF SCIENCES TARBIAT MODARRES UNIVERSITY, TEHRAN, IRAN
Introduction: To study the clastogenic effect of continuous ultrasound alone or in combination with procarbazine hydrochloride on new born rats bone marrow erythrocytes using micronucleus test.
Materials and Methods: Five days old new born rats were exposed to various ultrasound waves of 0.5, 1 and 2 W/cm2 and treated with different doses of 20, 40 and 80 mg/kg procarbazine hydrochloride. Samples of bone marrow were prepared after 24, 48 and 72 hour after ultrasound exposure, drug treatment alone or in combination. Following slide preparation, cells were stained in May's Grunwald-Giemsa. The frequency of micronuclei in polychromatic erythrocytes (PCE), and the ratio of PCE to total erythrocytes were determined.
Results: Results obtained from this study indicate that a continuous ultrasound wave used in the range of 0.5-2W/cm2, produces neither cytotoxic nor clastogenic effect on bone marrow erythrocytes. But when 2W/cm2 ultrasound wave was used with 80 mg/kg procarbazine, a moderate synergistic clastogenic effect was observed without being cytotoxic to cells.
Conclusion: There is moderate synergistic effect induced in erythrocytes following combined treatment of procarbazine and ultrasound waves, the mechanism is not fully understood yet. It seems that ultrasound effects on cytoplasmic membrane of cells lead to an increase in the intracellular concentration of drug and stimulate free radical production.