ANATOMY DEPARTMENT, TEHRAN MEDICAL SCIENCES UNIVERSITY
Abstract
Introduction: Bone marrow contains a population of stem cells capable of differentiating to osteoblast and forming the bone nodule by dexamethasone. Material and Methods: The stromal cells of bone marrow obtained from 4 to 6 weeks old Spruge-Dawely male rats were grown in primary culture for 7 days and subcultured for 18 days. The cells were cultured in either DMEM medium containing 15% fetal calf serum and antibiotics as the controls or the above medium supplemented with osteogenic supplements (OS): include 10 mM Na-beta glycerophosphate (Na-betaGp), 10 nM dexamethasone (Dex) and 50 g/ml ascordic acid (AsA) as the examined cultures. After 6, 12 and 18 days of grow up in subculture, the cultures were examined for mineralization and alkaline phosphatase (Apase) expression. Results: Mesenchymal stem cells (MSCs) in examined cultures underwent a dramatic change in cellular morphology and a significat increase in Apase activity by day 12. The deposition of a calcified matrix on the surface of the culture flasks became evident between days 12 and 18. Conclusion: The addition of osteogenic supplements (OS) to MSCs cultures induced Apase expression that contributes to cellular differentiation and mineralization of extracellular matrix.
Akbari, M., Nikbakht, M., & Sobhani, A. (2001). Expression Of Alkaline Phosphatase During Osteogenic Differentiation Of Rat Bone Marrow Stromal Cells. Cell Journal (Yakhteh), 3(3), 131-136.
MLA
M. Akbari; M. Nikbakht; A.Gh. Sobhani. "Expression Of Alkaline Phosphatase During Osteogenic Differentiation Of Rat Bone Marrow Stromal Cells". Cell Journal (Yakhteh), 3, 3, 2001, 131-136.
HARVARD
Akbari, M., Nikbakht, M., Sobhani, A. (2001). 'Expression Of Alkaline Phosphatase During Osteogenic Differentiation Of Rat Bone Marrow Stromal Cells', Cell Journal (Yakhteh), 3(3), pp. 131-136.
VANCOUVER
Akbari, M., Nikbakht, M., Sobhani, A. Expression Of Alkaline Phosphatase During Osteogenic Differentiation Of Rat Bone Marrow Stromal Cells. Cell Journal (Yakhteh), 2001; 3(3): 131-136.