Anatomy Department, Medical Faculty, Zahedan, Iran
Abstract
Introduction: Mucinous compounds are a group of neutral glycoprotein which have a prime effect for protection of surface epithelium against digestion. Studies showed the changes of these compounds in neoplasia and Intestinal metaplasia of the stomach. Materials and Methods: Specimens were taken from 25 surgically resected stomach from gastric lesion and it's periphery and processed as routine in pahology. Praffin blockes were cutted 3-5m thickness and sections were studied histochemically using Alcian blue pH=1 and pH=2.5, Toluidine blue pH=4.5 in Vernol's buffer,H&E and diffrent blocking techniques such as active metylation, mild metylation and active metylation with Saponification. Results: Results of this study showed the acidic nature of glycoprotein in intestinal metaplasia and signet ring cell carcinoma. More ever, sialomucins and sulphomucins were detected in type I and type IIb Intestinal metaplasia. Morever combination of tubidne blue and blocking technique showed that sulphate groups are responsible for metachromasia of mucinous compounds. Conclusion: The presence of sulphomucins in intestinl metaplasia recognized as arisk factor for progression of neoplasia , therfore recognition of these compounds inbiopsy specimen is a important warning and needs a serious follow up of the patient.
Arab, M., Altiraihi, T., & Shariat Torbaghan, S. (2001). Histochemical Study Of Mucous Substance Of Gastric Epithelial Lesions. Cell Journal (Yakhteh), 3(2), 65-70.
MLA
M.R. Arab; T. Altiraihi; Sh. Shariat Torbaghan. "Histochemical Study Of Mucous Substance Of Gastric Epithelial Lesions". Cell Journal (Yakhteh), 3, 2, 2001, 65-70.
HARVARD
Arab, M., Altiraihi, T., Shariat Torbaghan, S. (2001). 'Histochemical Study Of Mucous Substance Of Gastric Epithelial Lesions', Cell Journal (Yakhteh), 3(2), pp. 65-70.
VANCOUVER
Arab, M., Altiraihi, T., Shariat Torbaghan, S. Histochemical Study Of Mucous Substance Of Gastric Epithelial Lesions. Cell Journal (Yakhteh), 2001; 3(2): 65-70.