The Effect Of Reperfusion Ischemia On Level Vitamin E In Venous Blood And Kidney Tissue In Rat

Document Type : Original Article


Physiology Department, Tehran Medical Sciences University, Tehran, Iran


Introduction: During kidney and other organ's transplantation, the organ to be transplanted, must inevitably remain out of the body with little or no blood perfusion at all, for long period of time (Ischemia). This causes the release of oxygen free radicals (OFR). Reperfusion (reintroduction of blood flow) will further exacerbate the initial damage caused by the ischemic insult and may result in the production of free radicals. The goal of this study was to investigate the effect of ischemia- reperfusion (IR)(30 min ischemia followed by 10 min reperfusion)by clamping and decamping of the renal artery on the oxidant status of renal tissue and venous plasma.
Materials and Methods: White male rats with average weight of 26040 grams were randomly divided into sham-operated, control and ischemia-reperfusion (30 min ischemia, followed by 10 min reperfusion) groups (n= 7 each group). In the control group, 70 min after dissecting the abdomen, renal blood and tissue were removed. In the IR group, 30 min after dissecting the abdomen, 30 min ischemia (clamping of renal artery) and 10 min reperfusion (declamping of renal artery) were applied, then, the kidney and blood were sampled. Vitamin E (as an endogenous antioxidant) was extracted from the right kidney tissue and the venous plasma and measured by HPLC.
Results: The amount of vitamin E (َmol/L) in plasma of IR group had a significant decrease in respect to the control group (P<0.002). The same was true for kidney tissue (P<0.0004).
Conclusion: This study suggests that during reperfusion, the body uses all its defence mechanism to fight against the produced OFR the measured vitamin E showed the oxidative state of an organ (in this case kidney) wich would be of benefit.