Developmental Potetial Of Mouse Morula Early And Late Blastocyst After Vitrification

Document Type : Original Article




Introduction: The objective of this study was to assess developmental potential of mouse morulae, early and laic blastocysts after vitrification in R2 and R2+Vero mediums.
Material and Methods: Morulae, early and late blastocysts were obtained from superovulated NMRI female mice. Embryos in experimental group were suspended in a solution of Ethylene glycol, ficoll and sucrose (EFS40) for 2 minutes and were then loaded into 0.25 ml straws, holding them on liquid nitrogen vapour for 3-5 minutes, finally were immersed into liquid nitrogen. Embryos were thawed and equilibrated in 0.5 mol sucrose solution for 5 minutes, were cultured in R2 or R2+Vero mediums for I 20 hours.
Results: Results indicate high survival rate or embryos after vitrification (95, 85, 76% for morulae, early and late blastocysts respectively). Sixty three, 70 and 75% in R2 and 67, 73, 80% in R2+Vero reached to hatching and hatched blastocyst alter 4.S hours Respectively for morulae, early and late blastocyst of control groups. While 24, 19 and 12% in R2, and 18, 22 and 16% in R2+Vero of vitrified embryos were reached to Hatching and hatched blastocyst after 4S hours. Sixty five, 58 and 44% in R2 and 70, 63 and 56% in R2+Vero of vitrified embryos following a delay of 48 hours on the 4th of cultivation day reached lo hatching and hatched blastocyst.
Conclusion: Our results indicate that R2 and R2+Vero have the identical property lo support development of vitrified and non-vitrified embryos. Development of vitrified embryos show the same development competence comparing to control group alter a delay of 48 hours, from this statement we conclude that although vitrification damage the embryos Inn most of these damage are reversible in a suitable medium is less expensive and more convenient than R2+Vero.