The Role Of Adenosine A1 Receptor Activity Of Piriform Cortex Neurons On Amygdala Kindled Seizures In Rats

Document Type : Original Article


Tarbiat Modarres Unive~sity, Tehran, I


Objective: Considering the anticonvulsant effects of A1 Adenosine Receptors and the anatomical connections between pJriform Cortex and Amygdala, in this study, the role of A1 adenosine receptors activity of piriform cortex neurons on amygdala kindled seizures was investigated in rats.
Material and Methods: The rats were fully kindled by daily electrical stimulation of amygdala. N6-cyclohexyleadenosine (CHA; 1,10 and 100 /lM), as a selective A1 agonist and 1,3-dimethyl-8-cyclohexylexantine (CPT; 20 and 10 /lM), as a selective A1 antagonist were microinjected (0.5/l1, 0.25 /ll/min) into the piriform cortex. Animals were stimulated at 5, 15 or 90 min after drug microinjection and seizure parameters were measured.
Results: Intra-piriform CHA (10 or 100 /lM) reduced afterdischarge duration and stage 5 seizure duration and prolonged stage 4 latency significantly. Pretreatment with CPT (10 /lM) 5 min before CHA (100 /lM) eliminated the effects of CHA.
Conclusion: These observations suggest that A1 adenosine receptors activity in piriform cortex reduced the seizure severity and attenuated the distribution of seizure activity to other brain regions.