The Effect Of Granulocyte-Macrophase Colony Stimulating Factor On Development And Quality Of Murine Two-Cell Embryos

Document Type : Original Article



Introduction: Murine preimplantation embryos expressed Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) receptor and it is known as a growth factor. In this study capacity of two cell mouse embryos were studied in the presence and absence of GM-CSF.
Material and Methods: Female NMRI mice were superovulated using routine technique. 299 2-cell embryos were cultured in one group as the control (T6 medium supplemented with 5mg/ml bovine serum albumin) and in the other group as experimental (T6 medium supplemented with 5mg/ml bovine serum albumin and 2 ng/ml GM-CSF). Developmental rate of embryos was assessed daily. The blastocysts were stained and their total cell number were counted. The diameter of blastocysts were measured using calibrated eye piece.
Results: The cleavage rate of embryos in the presence of GM-CSF was increased during 48 and 72 hours after culturing. The blastocyst formation rate in the control and GM-CSF groups were 65.78 % and 74.82% and the diameter of blastocysts in these groups were 125.40 and 128.02 micrometer, respectively. Also total cell number of blastocysts in previous groups were 74.20 and 80. There was statistically significand differences between these groups in regard to blastocyst diameter and total cell number.
Conclusion: It seems the GM-CSF is a suitable embryotrophic factor.