MOLECULAR BIOLOGY RESEARCH CENTER, BAQIYATALLAH (A.S) UNIVERSITY OF MEDICAL SCIENCES, TEHRAN, IRAN
Abstract
Introduction: The aim of this study was to setup, optimize and introduce a sensitive and specific PCR detection method for identification of Neisseria meningitidis DNA in clinical samples. Material and Methods: Capsular transport gene A (ctrA) was selected as a specific target sequence. This primer pair amplifies 101bp of the target gene. Neisseria meningitidis strain: ATCC; 13090 and Neisseria meningitides serogroup C were used as a standard organism for optimization experiments. A range of bacterial pathogens were used for specificity testing, including, Haemophilus influenzae Type b: ATCC; 49766, Escherichia coli: ATCC;35218, Enterobacter, Klebsiella pneumoniae, Streptococcus pneumoniae, Staphylococcus aureus and Streptococcus group D. Phenol – chloroform method was used for DNA extraction. Amplified product was detected by gel agarose electrophoresis, stained by ethidiome bromide. Results: Our results confirmed amplification of the expected product. Specificity test proved no cross reaction with tested organisms. Sensitivity test detected 500fg of Neisseria meningitidis DNA as a final detection limit. Conclusion: As a conclusion, PCR is a method with high sensivity and specificity and specificity which can be performed within 3 hours, and therefore utilization of this test in the clinical laboratories can help rapid diagnosis of Neisseria meningitides in clinical samples.
Ataee, R., Ali Zadegan, M. G., Hajia, M., Tavana, A. M., Godarzi, Z., & Nakhjavani, F. (2006). Rapid Diagnosis Of Neisseria Meningitides By PCR Method. Cell Journal (Yakhteh), 8(2), 92-97.
MLA
R.A. Ataee; M. Gorban Ali Zadegan; M. Hajia; A. Mehrabi Tavana; Z. Godarzi; F. Nakhjavani. "Rapid Diagnosis Of Neisseria Meningitides By PCR Method". Cell Journal (Yakhteh), 8, 2, 2006, 92-97.
HARVARD
Ataee, R., Ali Zadegan, M. G., Hajia, M., Tavana, A. M., Godarzi, Z., Nakhjavani, F. (2006). 'Rapid Diagnosis Of Neisseria Meningitides By PCR Method', Cell Journal (Yakhteh), 8(2), pp. 92-97.
VANCOUVER
Ataee, R., Ali Zadegan, M. G., Hajia, M., Tavana, A. M., Godarzi, Z., Nakhjavani, F. Rapid Diagnosis Of Neisseria Meningitides By PCR Method. Cell Journal (Yakhteh), 2006; 8(2): 92-97.