IMMUNOLOGY DEPARTMENT, FACULTY OF MEDICINE, SHAHID BEHESHTI UNIVERSITY, TEHRAN, IRAN
Cell Banking is long –term storing of cell stocks that requires intensive care and maintenance to ensure its adequate quality. The main goal of a biological bank is preparing a repository source of cell culture system in a global manner. On the other hand, cell culture is an invaluable tool for investigators in numerous fields. Successful maintenance of cells requires knowledge and practice of some essential techniques. Many areas of medical research and therapeutic programs have benefited from the use of well characterized cell lines and tissue bankable cells. According to above mentioned, initiating material and safety aspects of handling cell lines is most important in controlling freezing rate. Through another section of this article, extensive discussion about mechanisms of biological characterization of cell population is defined . Some of the techniques about in vitro manipulation for complete processing of banked cells for improving proliferative capacities, culture longevity, and stability in various source of cells have been discussed in terms of quality control and banking procedures. For highly standardized procedures we recommended some quality control of cell line identity and methods for eradication of mycoplasma contamination and other microbial agents, since they have multitude effects on cell banks.