Immunology and Microbiology Department, Shaheed Beheshti University of Medical Sciences
The dorsal skin of the Iranian frog, Rana ridibanda, is associated with numerous prominent granular glands which extract their secretions in the response to stressor or invading pathogens .The secretions have broad spectrum antimicrobial effects. In this research the effect of antimicrobial skin secretions from Iranian frog (Rana ridibanda) has been examined against gram positive Methycillin Resistant Staph Aureus (MRSA), under sterile conditions. To show this effect, 1cm² of frog skin was cultured in complete tissue culture medium containing RPMI, FBS and FUNGISON for the period of 10 days. Immediately after, MRSA was exposed to frog skin secretion (medium culture) and Vancomycin. The results showed that the frog skin secretions has significant antimicrobial effect against MRSA. The range of inhibition zone for MRSA was the same as (20mm) Vancomycin in DISK method. In Minimum Inhibitory Concentration method, for MRSA, the tube 1/8 was positive.