A New Isolate Of The Genus Malassezia Based On The Sequence Analysis Of 26S And ITS1 In Ribosomal DNA


1 Medical Parasitology and Mycology Department, School of Public Health and Institute of Public Health Researches, Tehran University of Medical Sciences, Tehran, IRAN

2 Azad University, Khorasgan, Isfahan, Iran

3 Institute of Medical Mycology and Genome Research Center, Teikyo University, Tokyo, Japan


alassezia species considered to be the etiological agents of pityriasis versicolor and Malassezia follicolitis in humans. Recently, on the basis of molecular data, four new species were added to the genus. In total, 11 species have been described and accepted so far. In this study we describe a new isolate of Malassezia based on the nucleotide sequence of 26SrDNA and ITS1 regions, as the accepted critical markers for description of the species. The yeast was isolated from a hamster. Two primer pairs, one for amplification of D1/D2- 26Sr DNA and another for the ITS1 region were used in PCR. The PCR products were sequenced and analyzed to compare with other similar sequences which are already deposited in the GenBank. The 26SrDNA PCR product was also digested with the restriction enzyme CfoI. Malassezia-specific universal primer pairs successfully amplified the 26srDNA and ITS1 regions of the new isolate, providing a single PCR product of about 580 and 280 base pairs, respectively. After digestion of the 26s PCR product with the enzyme CfoI, a unique and different RFLP pattern was observed. Sequence analysis of D1/D226s and ITS1 regions were compared with the same regions in all already described Malassezia species, which implied a different and unique new sequences. The phylogenetic tree of both regions showed that the isolate could be a different Malassezia isolate. Regarding the new RFLP pattern of D1/D226SrDBA and the unique nucleotide sequence of both D1/D2 26SrDNA and ITS1 regions, we propose the isolate to be a new Malassezia.